Regulatory

Part:BBa_K1826000:Design

Designed by: Jayaprakash S   Group: iGEM15_SVCE_Chennai   (2015-09-18)


Mutant pBAD/araC promoter containing SacI site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

The promoter was constructed for cloning into pET 24a vector. A silent mutation was introduced in the base number 430 of the Biobrick part BBa_I0500. The base C was substituted with G without bringing any changes to the araC protein encoded in the 3' - 5' direction.

Source

The part was derived from the sequence of BBa_I0500 and partially ordered from IDT.

References